Cytoplasmic Buffer (Tris-Based) - 100ml
Components: Tris Base / Tris-HCl(10 mM), Sucrose(0.34 M), Calcium Chloride Dihydrate(3 mM), Magnesium Chloride Hexahydrate(2 mM), EDTA(0.1 mM), Nonidet P-40 Substitute(0.5%), Water(rest) - Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. - pH: 8.0 ± 0.15 - Storage: 2-8° C
